Thermo Fisher Scientific TIF1 Alpha/TRIM24 Polyclonal Antibody
TIF1 Alpha/TRIM24 단백질을 검출하기 위한 Thermo Fisher Scientific의 Rabbit Polyclonal 항체. Western blot, IHC, IP, ChIP-Seq 등 다양한 응용에 적합. 항원 친화 크로마토그래피로 정제되었으며, 4°C에서 보관. 연구용으로만 사용 가능.
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Applications and Tested Dilutions
| Application | Tested Dilution / Amount |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:200–1:1,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
| ChIP-sequencing (ChIP-Seq) | 4 µg/30 µg chromatin |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 700 and 750 of Human Transcriptional Intermediary Factor 1 Alpha (Tripartite Motif-Containing 24) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Wet ice |
Product Specific Information
- Recommended shelf life: 1 year from date of receipt
- For IHC: Epitope retrieval with citrate buffer (pH 6.0) is recommended for FFPE tissue sections.
Target Information
The protein encoded by this gene mediates transcriptional control by interacting with the activation function 2 (AF2) region of several nuclear receptors, including estrogen, retinoic acid, and vitamin D3 receptors. It localizes to nuclear bodies and associates with chromatin and heterochromatin-associated factors. This protein belongs to the tripartite motif (TRIM) family, characterized by three zinc-binding domains (RING, B-box type 1, and B-box type 2) and a coiled-coil region. Two alternatively spliced transcript variants encoding different isoforms have been described.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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