Thermo Fisher Scientific NCF4 Polyclonal Antibody
Human NCF4 단백질을 인식하는 Goat Polyclonal Antibody로, Western blot 및 IHC(P) 검증 완료. 합성 펩타이드 면역원 사용, 0.5 mg/mL 농도 액상 형태. TBS buffer(pH 7.3)에 0.5% BSA 및 0.02% sodium azide 포함. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.3–1 µg/mL |
| Immunohistochemistry (Paraffin) (IHC (P)) | 2–4 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide sequence (KDFPEEDDPTN) corresponding to the internal amino acids of NCF4 (aa 228–239) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Ammonium sulfate precipitation |
| Storage Buffer | TBS, pH 7.3, with 0.5% BSA |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_10987421 |
Product Specific Information
- This antibody is predicted to react with canine based on sequence homology.
- Tested in Peptide ELISA: antibody detection limit dilution 32,000.
Target Information
The protein encoded by this gene is a cytosolic regulatory component of the superoxide-producing phagocyte NADPH-oxidase, a multicomponent enzyme system important for host defense.
This protein is preferentially expressed in cells of myeloid lineage. It interacts primarily with neutrophil cytosolic factor 2 (NCF2/p67-phox) to form a complex with neutrophil cytosolic factor 1 (NCF1/p47-phox), which further interacts with the small G protein RAC1 and translocates to the membrane upon cell stimulation.
This complex then activates flavocytochrome b, the membrane-integrated catalytic core of the enzyme system. The PX domain of this protein can bind phospholipid products of PI(3) kinase, suggesting its role in PI(3) kinase-mediated signaling events. The phosphorylation of this protein negatively regulates enzyme activity. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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