
Thermo Fisher Scientific Beclin 1 Polyclonal Antibody
Beclin 1 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ELISA, IP 등 다양한 응용 가능. 인간, 생쥐, 랫트 반응성. 자가포식 관련 연구에 적합하며 고순도 Affinity Chromatography로 정제됨.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:100–1:500 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5–4 µg antibody for 200–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1–280 of human Beclin 1 (NP_0037571) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.23 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | −20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2808451 |
Product Specific Information
Positive Samples: U-87MG, HeLa, MCF7, Mouse spleen, Rat ovary, Rat lung
Cellular Location: Cytoplasm, Cytoplasmic vesicle, Endoplasmic reticulum membrane, Endosome, Endosome membrane, Golgi apparatus, Mitochondrion, Mitochondrion membrane, Nucleus, Peripheral membrane protein, Autophagosome, Trans-Golgi network membrane
Immunogen Sequence:
MEGSKTSNNS TMQVSFVCQR CSQPLKLDTS FKILDRVTIQ ELTAPLLTTA QAKPGETQEE ETNSGEEPFI ETPRQDGVSR RFIPPARMMS TESANSFTLI GEASDGGTME NLSRRLKVTG DLFDIMSGQT DVDHPLCEEC TDTLLDQLDT QLNVTENECQ NYKRCLEILE QMNEDDSEQL QMELKELALE EERLIQELED VEKNRKIVAE NLEKVQAEAE RLDQEEAQYQ REYSEFKRQQ LELDDELKSV ENQMRYAQTQ LDKLKKTNVF NATFHIWHSG
Target Information
Beclin 1은 자가포식 과정에서 중심적인 역할을 하는 단백질로, PI3K 복합체의 핵심 서브유닛으로 작용하여 phosphatidylinositol 3-phosphate 형성을 매개합니다. PI3KC3-C1 복합체는 자가포식소체의 개시 단계에, PI3KC3-C2 복합체는 성숙 및 엔도사이토시스 단계에 관여합니다. 또한 Beclin 1은 항바이러스 숙주 방어에도 중요한 역할을 합니다.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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