Thermo Fisher Scientific CDC45 Monoclonal Antibody (OTI4F3), TrueMAB
CDC45 단백질을 표적하는 Mouse 모노클로날 항체로, Western blot 및 IHC(P)에서 검증됨. Human 시료에 반응하며, 액상 형태로 1 mg/mL 농도. Affinity chromatography로 정제되었으며, -20°C에서 보관 권장. 연구용으로만 사용 가능.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:500 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI4F3 |
| Immunogen | Human recombinant protein fragment corresponding to amino acids 1–270 of human CDC45 produced in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 1% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The protein encoded by this gene shows strong similarity to Saccharomyces cerevisiae Cdc45, an essential protein required for the initiation of DNA replication. Cdc45 is part of a conserved multiprotein complex with Cdc6/Cdc18, minichromosome maintenance proteins (MCMs), and DNA polymerase, which are crucial for early steps of DNA replication in eukaryotes. It interacts with MCM7 and DNA polymerase alpha, and studies in Xenopus suggest it plays a role in loading DNA polymerase alpha onto chromatin. Multiple polyadenylation sites have been reported.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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