Thermo Fisher Scientific Rat anti-Mouse IgM (Heavy chain) Secondary Antibody
Rat monoclonal anti-Mouse IgM (Heavy chain) 항체로, 다양한 면역학적 분석에 사용 가능. Mouse IgM에 특이적이며 IgG, IgA, light chain과 반응하지 않음. Western blot, IHC, Flow cytometry, ELISA에 적합. 고순도 정제된 액상 형태로 안정적 보관 가능.
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Applications and Tested Dilution
| Application | Dilution Range |
|---|---|
| Western Blot (WB) | 1:500–1:20,000 |
| Immunohistochemistry (IHC) | 1:500–1:20,000 |
| Flow Cytometry (Flow) | 1:500–1:20,000 |
| ELISA | 1:500–1:20,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Secondary Antibody |
| Clone | LO-MM-9 |
| Immunogen | Mouse IgM |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 0.1% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2532954 |
| Target | IgM (Heavy chain) |
| Antibody Form | Whole Antibody |
Product Specific Information
This monoclonal Rat anti-Mouse IgM reacts specifically with the mouse IgM heavy chain and shows no cross-reactivity with mouse IgG subclasses, IgA, or light chains.
Target Information
Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins. They are useful for detection, sorting, or purification of specific targets. These antibodies provide versatility across multiple detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. Typically, secondary antibodies are generated by immunizing the host animal with pooled immunoglobulins from the target species, followed by purification and optional modifications such as immunoaffinity chromatography, antibody fragmentation, or label conjugation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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