Thermo Fisher Scientific WDR48 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:100

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 450-677 of human WDR48 (NP_0658901) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:WDR48,uniProtId:Q8TAF3-1,ncbiNodeId:9606,antigenRange:450-677,antigenLength:677,antigenImageFileName:PA5-117073_WDR48_Q8TAF3-1_Rabbit.svg,antigenImageFileNamePDP:PA5-117073_WDR48_Q8TAF3-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.7 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.02% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2901703

Product Specific Information

Positive Samples: U-251MG, A-431, SGC-7901, LO2, A-549, HT-1080, Mouse brain, Rat testis

Immunogen sequence: FSSPDGSDPK LNLGGLLLQA LLEYWPRTHV NPMDEEENEV NHVNGEQENR VQKGNGYFQV PPHTPVIFGE AGGRTLFRLL CRDSGGETES MLLNETVPQW VIDITVDKNM PKFNKIPFYL QPHASSGAKT LKKDRLSASD MLQVRKVMEH VYEKIINLDN ESQTTSSSNN EKPGEQEKEE DIAVLAEEKI ELLCQDQVLD PNMDLRTVKH FIWKSGGDLT LHYRQKST

Target Information

WD-repeats are motifs that are found in a variety of proteins and are characterized by a conserved core of 40-60 amino acids, which commonly form a tertiary propeller structure. While proteins that contain WD-repeats participate in a wide range of cellular functions, they are generally involved in regulatory mechanisms involving signal transduction, apoptosis, transcriptional regulation and cell cycle control. WD repeats serve as sites for protein-protein interaction and some seem to mediate the assembly of protein complexes. With eight WD repeats, WDR48 (WD repeat-containing protein 48), also known as USP1-associated factor 1 and p80, is a 677 amino acid protein that functions to regulate deubiquitinating complexes via activation of USP1, USP12 and USP46. WDR48 enhances deubiquitination by increasing catalytic turnover without increasing the affinity of deubiquitinating enzymes for the substrate. WDR48 is ubiquitously expressed and is mainly localized to the cytoplasm. There are five isoforms of WDR48 that are expressed as a result of alternative splicing events.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.