Thermo Fisher Scientific PICK1 Recombinant Rabbit Monoclonal Antibody (JG30-80)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:2,000-1:5,000

-

Flow Cytometry (Flow)

1:50-1:100

-

Immunoprecipitation (IP)

1:10-1:50

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JG30-80

Immunogen

Synthetic peptide within Human PICK1 aa 1-50 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:PICK1,uniProtId:Q9NRD5-1,ncbiNodeId:9606,antigenRange:1-50,antigenLength:415,antigenImageFileName:MA5-34772_PICK1_Q9NRD5-1_Rabbit.svg,antigenImageFileNamePDP:MA5-34772_PICK1_Q9NRD5-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Shipping conditions

Wet ice

RRID

AB_2848680

Product Specific Information

Positive Control: SK-Br-3, 293T.

Target Information

PSD-95/DLG/ZO-1 (PDZ) domain-containing proteins play a central role in synaptic membrane protein localization. The protein interacting with protein kinase C (PICK1) is a synaptic PDZ domain protein that also contains a coiled-coil and acidic domain. Studies indicate that PICK1 functions as a targeting and transport protein and has been shown to interact with protein kinase C alpha (PKCalpha), AMPA-type glutamate receptors, and several other membrane receptors via its PDZ domain. The interaction of PICK1 with PKCalpha is highly dependent on the activation of the kinase. It appears that PICK1 directs the activated form of PKCalpha to membrane anchored GluR2. Once phosphorylated by PKCalpha, GluR2 is released from the synaptic anchor proteins. Once released, GluR2 is transported from the synaptic membrane in a PICK1-dependent manner.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.