Thermo Fisher Scientific PIST Recombinant Rabbit Monoclonal Antibody (JG79-35)
Thermo Fisher Scientific의 PIST Recombinant Rabbit Monoclonal Antibody (JG79-35)는 인간 PIST 단백질을 인식하는 고특이성 항체입니다. Western blot, IHC, ICC/IF, Flow cytometry, IP에 적합하며, HEK293 발현 시스템에서 생산된 고순도 단클론 항체로 연구용에 최적화되어 있습니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| Flow Cytometry (Flow) | 1:50–1:100 |
| Immunoprecipitation (IP) | 1:10–1:50 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JG79-35 |
| Immunogen | Recombinant protein within Human PIST aa 1–140 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles, store in dark |
| Shipping Conditions | Wet ice |
| RRID | AB_2848679 |
Product Specific Information
Positive Control: SiHa, HepG2, MCF-7, SKOV-3, human liver cancer tissue, human colon tissue, human kidney tissue, mouse brain tissue.
Target Information
PDZ domains contain approximately 90 amino acids and bind the extreme C terminus of proteins in a sequence-specific manner.
PIST, a PDZ domain-containing Golgi protein, was discovered in a yeast two-hybrid system as a binding partner to Beclin-1, a Bcl-2-interacting protein homologous to the yeast autophagy gene apg6.
Experiments with mutant PIST proteins lacking the PDZ domain showed that PIST interaction with Beclin-1 through its coiled-coil domain can modulate Beclin-1 activity and suggest that PIST interactions with other proteins through its PDZ domain may regulate the activity of PIST and Beclin-1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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