Thermo Fisher Scientific ADAR1 Recombinant Rabbit Monoclonal Antibody (JU99-33)

Applications and Tested Dilutions

Product Specifications

Product Specific Information

Positive Control: Hela, human lung cancer tissue, human colon cancer tissue, human kidney tissue, human pancreas tissue, SiHa.

Target Information

Adenosine Deaminase RNA Specific (ADAR) catalyzes the hydrolytic deamination of adenosine to inosine in double-stranded RNA (dsRNA), referred to as A-to-I RNA editing. This process can affect gene expression and function in multiple ways, including:

• Altering mRNA translation by changing codons and amino acid sequences
• Modifying pre-mRNA splicing through splice site recognition changes
• Affecting RNA stability via nuclease recognition sequence modification
• Influencing genetic stability in RNA virus genomes
• Regulating RNA structure-dependent activities such as microRNA production and protein-RNA interactions

ADAR can edit both viral and cellular RNAs, either at multiple (hyper-editing) or specific sites. Cellular RNA substrates include BLCAP, GRIA2, HTR2C, and GABRA3, where site-specific RNA editing alters protein function.

Viral RNA substrates include HCV, VSV, MV, HDV, and HIV-1. ADAR1 exhibits either proviral or antiviral effects that may be editing-dependent or independent. For example:

• Inhibits HCV replication via RNA editing
• Enhances replication of MV, VSV, and HIV-1 via suppression of EIF2AK2/PKR activation
• Stimulates HIV-1 infectivity through editing in the 5′UTR and coding regions
• Enhances HDV replication by A-to-I editing at amber/W site, promoting large delta antigen synthesis

However, excessive ADAR1 expression can inhibit HDV replication.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.