Thermo Fisher Scientific STAT4 Monoclonal Antibody (OTI1B1), TrueMAB

Applications and Tested Dilutions

Product Specifications

Additional Formats

• Biotin
• Request custom conjugation

Product Specific Information

For reconstitution, add 100 µL distilled water to obtain a final antibody concentration of about 1 mg/mL.
For conjugation experiments, perform an additional desalting step (Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

STAT4 was originally identified using degenerate primers complementary to conserved regions of other STAT proteins. It shares 52% similarity with STAT1 and 47% with STAT3. STAT4 can be tyrosine phosphorylated by Jak1 or Jak2, forming homo- and heterodimers with other STAT family members. Tyrosine-phosphorylated STAT4 binds the IFN-gamma activated site (GAS), and serine phosphorylation is required for maximal transcriptional activity.

STAT4 expression is restricted to thymus, spleen, and testis. It is activated by interleukin 12 (IL-12), which is essential for T-cell independent induction of IFN-gamma and Th1 response development. STAT4-deficient mice show impaired IL-12-mediated Th1 development and enhanced Th2 differentiation. In mice, IFN-α/β activation of STAT4 is required for IFN-γ production during viral infection.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.