Thermo Scientific™

HisProbe™-HRP Conjugate

Thermo Scientific HisProbe-HRP is a nickel (Ni2+)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins자세히 알아보기

Thermo Scientific HisProbe-HRP is a nickel (Ni2+)-activated derivative of horseradish peroxidase (HRP) that enables direct, IMAC-based detection of His-tagged proteins and other histidine-rich proteins in Western blots and microplates.

Features of HisProbe-HRP Conjugate:

• Fast—His-tag detection is achieved with a one-step probe incubation that eliminates the need to run a lengthy two-step primary/secondary antibody reaction protocol
• Economical—no need to purchase expensive primary/secondary antibodies; considerably less costly than anti-6xHis-tag antibodies
• High specificity—HisProbe-HRP can recognize his-tags independent of adjacent tags.
• Less steric hindrance—HisProbe-HRP is smaller than antibodies and detects his tagged fusion proteins that are often undetectable when using some anti-his-tag antibodies

Unlike anti-6xHis antibodies, HisProbe-HRP can recognize polyhistidine tags independent of adjacent tags. The active ligand in HisProbe-HRP is a tridentate chelator that allows Ni2+ to be bound in active form for subsequent interaction and detection fo target molecules. The active chelator has binding capabilities similar to iminodiacetic acid (IDA) when used for immobilized metal affinity chromatography (IMAC). The Thermo Scientific SuperSignal West HisProbe Kit includes blocking buffers and chemiluminescent substrate for sensitive Western blot detection via the HisProbe-HRP Conjugate.

Related Products
SuperSignal™ West HisProbe Kit

사양

설명Pierce HisProbe-HRP Conjugate

제품 유형HRP Conjugate

검출 방법Chemiluminescence

형태Solid

제품라인HisProbe™

수량2 mg

반응성All species

Unit SizeEach