Thermo Fisher Scientific Pan SHANK Monoclonal Antibody (N23b/49), APC
Pan SHANK 단백질을 인식하는 마우스 단일클론 항체로, APC 형광체가 결합되어 있습니다. WB, IHC, ICC/IF 등 다양한 응용에 적합하며 Shank1-4 단백질 검출에 유용합니다. 단백질 G로 정제되었으며 4°C에서 보관합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:1,000 |
| Immunohistochemistry (IHC) | 1:1,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | Assay-dependent |
| Antibody Microarray (AM) | Assay-dependent |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | N23b/49 |
| Immunogen | Fusion protein amino acids 84–309 of rat Shank2 |
| Conjugate | APC |
| Excitation / Emission Max | 651 / 660 nm |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein G |
| Storage Buffer | 95.64 mM phosphate / 2.48 mM MES, pH 7.4, with 0.5 M EDTA |
| Contains | No preservative |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2931892 |
Available Formats
- Unconjugated (MA5-27633)
- FITC (MA5-45439)
- PE (MA5-45441)
- PerCP (MA5-45440)
- Request custom conjugation
Product Specific Information
1 µg/mL of MA5-45438 was sufficient for detection of Shank1–4 in 10 µg of rat brain lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG:HRP as the secondary antibody. Detects approximately 160 kDa. Recognizes Shank1, Shank2, and Shank3.
Target Information
Shank proteins are a family of scaffold proteins interacting with various membrane and cytoplasmic proteins. They are located at postsynaptic sites of excitatory synapses and play a role in signal transmission into the postsynaptic neuron. Shank2 is expressed in developing retinal neurons and may be involved in neuronal differentiation. Disruption of glutamate receptors at the Shank postsynaptic platform may contribute to synaptic dysfunction and loss observed in Alzheimer’s disease.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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