Thermo Fisher Scientific ASH2L Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 81-280 of human ASH2L (NP_0010986841) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ASH2L,uniProtId:Q9UBL3-1,ncbiNodeId:9606,antigenRange:81-280,antigenLength:628,antigenImageFileName:PA5-89896_ASH2L_Q9UBL3-1_Rabbit.svg,antigenImageFileNamePDP:PA5-89896_ASH2L_Q9UBL3-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1.02 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.01% thimerosal

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2805828

Product Specific Information

Immunogen sequence: NLTWQSRTQD EHPKTMFSKD KDIIPFIDKY WECMTTRQRP GKMTWPNNIV KTMSKERDVF LVKEHPDPGS KDPEEDYPKF GLLDQDLSNI GPAYDNQKQS SAVSTSGNLN GGIAAGSSGK GRGAKRKQQD GGTTGTTKKA RSDPLFSAQR LPPHGYPLEH PFNKDGYRYI LAEPDPHAPD PEKLELDCWA GKPIPGDLYR; Positive Samples: K-562, HepG2; Cellular Location: Nucleus

Target Information

The human ASH2L gene encodes a 628 amino acid protein known as ASH2L1, or isoform 1, which contains a nuclear localization signal and PHD finger motif, suggesting that the gene product functions as a transcription regulator. Its predicted molecular weight is 68.7kDa. Alternative splicing results in possible isoforms at 60 kDa & 56.5 kDa). Human ASH2L proteins are 60% homologous to Drosophila ash2, which positively regulates expression of certain genes in early development, and contain similar, but not identical, domains, including a zinc finger motif. Homology to mouse ASH2L is 90%. ASH2L is predominantly expressed in adult heart and testis and fetal lung and liver, with barely detectable expression in adult lung, liver, kidney, prostate, and peripheral leukocytes as well as leukemia cell lines with erythroid and megakaryocytic potential, such as K562, Hel and Dami. Differentiation inducers (e.g. phorbol ester and hemin) cause different expression patterns in these cell lines, suggesting that ASH2L plays a role in hematopoiesis and is associated with particular types of leukemia. In addition ASH2L has been suggested to interact with beta-tubulin and beta-catenin.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.