Thermo Fisher Scientific KARS Recombinant Rabbit Monoclonal Antibody (ARC1765)
KARS 단백질을 표적하는 Recombinant Rabbit Monoclonal Antibody로, WB, IHC, ICC, ELISA, IP 등 다양한 응용에 적합합니다. 인간, 생쥐, 랫트 반응성을 가지며 HEK293 세포에서 발현된 고순도 항체입니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC-P) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
| Immunoprecipitation (IP) | 0.5 µg–4 µg antibody for 200 µg–400 µg extracts |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | ARC1765 |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 500–597 of human LysRS/KARS (Q15046) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.6 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 0.05% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2898088 |
Product Specific Information
- Positive Samples: HeLa, 293T, C6, Mouse testis, Rat testis
- Immunogen sequence:
TELNDPMRQR QLFEEQAKAK AAGDDEAMFI DENFCTALEY GLPPTAGWGM GIDRVAMFLT DSNNIKEVLL FPAMKPEDKK ENVATTDTLE STTVGTSV
Target Information
Aminoacyl-tRNA synthetases are enzymes that charge tRNAs with their cognate amino acids. Lysyl-tRNA synthetase (KARS) is a cytoplasmic homodimer belonging to the class II family of tRNA synthetases. It is known as a target of autoantibodies in polymyositis and dermatomyositis. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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