Thermo Fisher Scientific Mouse anti-Rat Kappa Light Chain Secondary Antibody, Biotin
Mouse monoclonal anti-Rat Kappa Light Chain antibody conjugated with biotin. Suitable for WB, Flow Cytometry, and ELISA. High specificity and purity with 0.5 mg/mL concentration. For research use only.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Flow Cytometry (Flow) | 1 µg/1×10⁶ cells |
| ELISA | 1:5,000–1:10,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Mouse, Rat |
| Host / Isotype | Mouse / IgG2a, kappa |
| Class | Monoclonal |
| Type | Secondary Antibody |
| Clone | K4F5 |
| Immunogen | This mouse IgG1-kappa class-switch variant monoclonal antibody reacts with rat kappa light chains and can be used for identification and enumeration of rat kappa light chain⁺ cells by flow cytometry. |
| Conjugate | Biotin |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Purification | Purified |
| Storage Buffer | PBS, pH 7.4 |
| Contains | <0.1% sodium azide |
| Storage Conditions | 4°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2534440 |
| Target | Kappa light chain |
| Antibody Form | Whole Antibody |
Additional Formats
- FITC
- PE
- Custom conjugation available upon request
Target Information
Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins. They are useful for detection, sorting, or purification of specific targets. Secondary antibodies provide versatility for various detection systems (e.g., HRP, AP, fluorescence) and enhance sensitivity through signal amplification. They are typically generated by immunizing the host animal with pooled immunoglobulins from the target species and may be further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to ensure high specificity and performance.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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