Thermo Fisher Scientific Mouse anti-Rat Kappa Light Chain Secondary Antibody, Biotin

Applications

Tested Dilution

Western Blot (WB)

1:2,000-1:10,000

Flow Cytometry (Flow)

1 µg/1x10^6 cells

ELISA (ELISA)

1:5,000-1:10,000

Product Specifications

Species Reactivity

Mouse, Rat

Host/Isotype

Mouse / IgG2a, kappa

Class

Monoclonal

Type

Secondary Antibody

Clone

K4F5

Immunogen

This mouse IgG1-kappa class-switch variant monoclonal antibody reacts with rat kappa light chains and can be used for identification and enumeration of rat kappa light chain+ cells by flow cytometry.

Conjugate

Biotin Biotin Biotin

View additional formats

• [FITC](/antibody/secondary/product-name/Rat Kappa Light Chain/conjugates/FITC/host/Mouse/)
• [PE ](/antibody/secondary/product-name/Rat Kappa Light Chain/conjugates/PE/host/Mouse/)
• Request custom conjugation

Form

Liquid

Concentration

0.5 mg/mL

Purification

purified

Storage buffer

PBS, pH 7.4

Contains

<0.1% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2534440

Target

Kappa light chain

Antibody Form

Whole Antibody

Target Information

Anti-Rat secondary antibodies are affinity-purified antibodies with well-characterized specificity for rat immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.