Thermo Fisher Scientific Phospho-IRF3 (Ser386) Polyclonal Antibody

Applications and Tested Dilutions

Product Specifications

Product Specific Information

This antibody detects endogenous levels of IRF3 only when phosphorylated at Serine 386.

Target Information

IRF3 (interferon regulatory factor 3) is a key transcriptional regulator of type I interferon (IFN)-dependent immune responses. It plays a critical role in the innate immune response against DNA and RNA viruses. IRF3 regulates the transcription of type I IFN genes (IFN-alpha and IFN-beta) and IFN-stimulated genes (ISG) by binding to an interferon-stimulated response element (ISRE) in their promoters. It acts as a more potent activator of the IFN-beta gene than the IFN-alpha gene and is involved in both early and late phases of IFNA/B gene induction. IRF3 is found in an inactive form in the cytoplasm of uninfected cells and, following viral infection or TLR signaling, is phosphorylated by IKBKE and TBK1 kinases. This induces a conformational change leading to dimerization, nuclear localization, and association with CREB binding protein to form dsRNA-activated factor 1 (DRAF1), which activates transcription of type I IFN and ISG genes. Mutations in this gene can result in acute infection-induced encephalopathy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.