
Thermo Fisher Scientific Mouse anti-Human IgE Secondary Antibody
인간 IgE를 인식하는 Mouse monoclonal 2차 항체로 Western blot, Flow cytometry, ELISA에 사용 가능. 비결합형(Unconjugated) 형태이며 Protein A로 정제됨. 고특이성과 신호 증폭에 유용. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2000 |
| Flow Cytometry (Flow) | 4 µg/mL |
| ELISA | Assay-dependent |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Secondary Antibody |
| Clone | 4H10 |
| Immunogen | Purified human IgE |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | PBS, pH 7.4 |
| Contains | 15 mM sodium azide |
| Storage Conditions | 4°C, do not freeze |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_1075895 |
| Target | IgE |
| Antibody Form | Whole Antibody |
Product Specific Information
This antibody recognizes an epitope different from those recognized by BE5 and 4G7 antibodies to IgE. The epitope is located within amino acids 267–279 (TWLEDGQVMDVDL).
Target Information
Anti-Human secondary antibodies are affinity-purified antibodies with well-characterized specificity for human immunoglobulins. They are useful for detection, sorting, or purification of specific targets. Secondary antibodies enhance assay versatility and sensitivity through signal amplification, as multiple secondary antibodies can bind to a single primary antibody. Typically, these antibodies are generated by immunizing host animals with pooled immunoglobulins from the target species and further purified or modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation) to produce highly specific reagents.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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