Thermo Fisher Scientific Phospho-MYPT1 (Thr853) Polyclonal Antibody
MYPT1 단백질의 Thr853 인산화 부위를 인식하는 Rabbit Polyclonal 항체. Western blot 및 IHC(Paraffin) 검증 완료. 액상 형태, 1 mg/mL 농도, -20°C 보관. 세포 수축 및 actin-myosin 상호작용 연구에 적합.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:500–1:1,000 | - |
| Immunohistochemistry (IHC) | - | View 1 publication |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:100 | - |
Product Specifications
| Specification | Description |
|---|---|
| Published species | Human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthetic phosphopeptide derived from human MYPT1 around the phosphorylation site of Thr853 (R-S-TP-G-V) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Storage conditions | -20°C |
| Shipping conditions | Wet ice |
| RRID | AB_2554900 |
Target Information
Myosin phosphatase target subunit 1 (MYPT1), also known as the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase.
Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho.
The active form of RhoA (GTP-RhoA) interacts with the myosin-binding subunit (MBS) of myosin phosphatase, regulating MLC phosphorylation.
Rho-associated kinase (Rho-kinase), activated by GTP-RhoA, phosphorylates MBS and inactivates myosin phosphatase.
Overexpression of RhoA or activated RhoA increases phosphorylation of MBS and MLC, indicating Rho inhibits myosin phosphatase via Rho-kinase.
Several transcript variants encoding different isoforms have been identified for this gene.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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