Thermo Fisher Scientific MECA-79 Monoclonal Antibody (MECA-79)

Applications

Tested Dilution

Publications

Western Blot (WB)

1-2 µg/mL

-

Immunohistochemistry (PFA fixed) (IHC (PFA))

1-2 µg/mL

-

Immunocytochemistry (ICC/IF)

1-2 µg/mL

-

Flow Cytometry (Flow)

1-2 µg/10^6 cells

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rat / IgM, kappa

Class

Monoclonal

Type

Antibody

Clone

MECA-79

Immunogen

Mouse lymph node stromal cells

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A/G

Storage buffer

PBS, pH 7.4

Contains

no preservative

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Ambient (domestic); Wet ice (international)

Target Information

HEV peripheral lymph node addressin (PNAd) is a glycoprotein is expressed on the luminal surface and in the cytoplasm of HEVs in lymphoid tissues, including peripheral and mesenteric lymph nodes and, to a lesser extent, Peyer`s patches. The MECA-79 antigen has also been detected on follicular B cells in the spleen and appendix in humans, as well as at sites of chronic inflammation. Post-capillary venules or large vessels in the spleen, thymus, or non-lymphoid tissues do not express the MECA-79 antigen and thus cannot be detected with this antibody. This addressin is involved in lymphocyte infiltration and adhesion into secondary lymphoid tissues.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.