Thermo Fisher Scientific AIF Recombinant Rabbit Monoclonal Antibody (4B2)
AIF 단백질을 인식하는 재조합 토끼 모노클로날 항체로, Western blot 및 IHC(Paraffin) 실험에 적합. 인간 및 랫트 반응성. HEK293 세포에서 발현된 IgG 형 항체로, 고순도 친화 크로마토그래피로 정제됨. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:5,000
- Publications: References
Immunohistochemistry (Paraffin) (IHC (P))
- Tested Dilution: 1:50–1:200
- Publications: References
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Rat |
| Host/Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | 4B2 |
| Immunogen | A synthesized peptide derived from human AIF (UniProt ID: O95831-1, antigen range: 516–532) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.17 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol, 150 mM NaCl |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C or -80°C if preferred |
| Shipping Conditions | Wet ice |
| RRID | AB_2898264 |
Product Specific Information
This antibody has been tested in direct ELISA.
Target Information
Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was identified and cloned as a key factor in apoptosis, characterized by nuclear changes such as chromatin condensation and fragmentation. During apoptosis, AIF translocates from mitochondria to the nucleus, promoting release of apoptogenic proteins like cytochrome c and caspase-9. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, leading to severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants, and a related pseudogene exists on chromosome 10.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
제품 이미지
(해당 항체의 항원 이미지: MA5-38350_AIF_O95831-1_Rabbit.svg, MA5-38350_AIF_O95831-1_Rabbit_PDP.jpeg)
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