Thermo Fisher Scientific Phospho-SF1 (Ser82) Polyclonal Antibody
Phospho-SF1 (Ser82) 폴리클로날 항체로 인간, 마우스, 비인간 영장류 반응성. Western blot, IHC, ICC/IF, ELISA에 사용 가능. 항원 친화 크로마토그래피로 정제된 고품질 항체로 안정적인 액상 형태 제공.
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Thermo Fisher Scientific Phospho-SF1 (Ser82) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (IHC) | 1:100–1:300 |
| Immunocytochemistry (ICC/IF) | 1:200–1:1,000 |
| ELISA | 1:5,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Non-human primate |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthesized peptide derived from human Splicing factor 1 around the phosphorylation site of S82 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage Buffer | PBS, pH 7.4, with 0.5% BSA, 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2607740 |
Product Specific Information
This antibody is predicted to react with mouse and monkey based on sequence homology.
Target Information
Splicing factor 1 (SF1) is a nuclear pre-mRNA splicing factor that specifically recognizes the intron branch point sequence at the 3′ splice site, together with the large subunit of U2 auxiliary factor (U2AF), and is required for the early stages of spliceosome assembly.
It also plays a role in nuclear pre-mRNA retention and transcriptional repression.
The protein contains an N-terminal U2AF ligand motif, a central hnRNP K homology motif and quaking 2 region, a zinc knuckles domain, and a C-terminal proline-rich domain.
Alternative splicing results in multiple transcript variants.
Usage Note
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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