Thermo Fisher Scientific Human RET [G691S], GST Tag, His Tag Recombinant Protein

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

658-1114

Tag

GST-tag, His-tag

Molecular weight

80.5 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM HEPES, pH 7.5, with 100mM NaCl, 5mM DTT, 15mM glutathione, 20% glycerol

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

Human RET G691S, C-terminal fragment, amino acids H658-S1114 (as in NCBI/Protein entry NP_066124.1) with a G691S mutation, N-terminal GST-HIS6 fusion protein with a 3C cleavage site, expressed in Sf9 insect cells.

• Validated in LanthaScreen™ Kinase Binding Assay
• Protocols available for simple use and optimized performance
• Multiple pack sizes available for convenient use

Related Product:
LanthaScreen™ Eu Kinase Binding Assay

Protein Form: Recombinant

Target Information

RET is a member of the cadherin superfamily, encodes one of the receptor tyrosine kinases which are cell-surface molecules that transduce signals for cell growth and differentiation. RET plays a crucial role in neural crest development, can undergo oncogenic activation in vivo and in vitro by cytogenetic rearrangement. Mutations in the RET gene are associated with the disorders multiple endocrine neoplasia, type IIA, multiple endocrine neoplasia, type IIB, Hirschsprung disease, and medullary thyroid carcinoma. Two transcript variants encoding different isoforms have been found for the RET gene. Additional transcript variants have been described for RET but their biological validity has not been confirmed.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.