Thermo Fisher Scientific Phospho-eNOS (Ser633) Polyclonal Antibody
Phospho-eNOS (Ser633) 특이적 폴리클로날 항체로, 인간, 마우스, 랫에서 반응. Western blot 및 Immunocytochemistry에 적합. 액상 형태, 1 mg/mL 농도, -20°C 보관. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:1,000–1:3,000
- Publications: References
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:500
- Publications: References
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human NOS3 (Accession P29474), corresponding to amino acid residues around phosphorylated Ser633 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Sequential chromatography |
| Storage Buffer | PBS, pH 7.4, with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2817247 |
Product Specific Information
Antibody detects endogenous levels of eNOS only when phosphorylated at Ser633.
Target Information
Nitric oxide (NO) is an inorganic, gaseous free radical that acts as a signaling molecule between cells. It is involved in vasorelaxation, neurotransmission, and cytotoxicity.
NO production is mediated by nitric oxide synthase (NOS) family members, which catalyze the oxidation of L-arginine to L-citrulline and NO.
Three NOS isoforms have been cloned:
- nNOS (type I, neuronal)
- eNOS (type III, endothelial)
- iNOS (type II, inducible)
All NOS isoforms contain calmodulin, NADPH, FAD, and FMN binding domains.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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