Thermo Fisher Scientific Influenza A H3N2 HA (A/Aichi/2/1968) His-tag Recombinant Protein

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

Immunohistochemistry (IHC)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Control (Ctrl)

Assay-dependent

-

Product Specifications

Species

Virus

Expression System

HEK293 cells

Amino acid sequence

Influenza A virus H3N2 (A/Aichi/2/1968) hemagglutinin, amino acids Met1-Trp530 (HA1+HA2, uncleaved) (Accession # AAA43178.1) with a C-terminal His-tag

Tag

His-tag

Molecular weight

59 kDa

Class

Recombinant

Type

Protein

Purity

>97% by SDS-PAGE

Endotoxin concentration

<1.0 EU/µg

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Lyophilized

Purification

purified

Storage buffer

20mM tris, pH 8, with 5% mannitol, 5% trehalose, 150mM NaCl, 10% glycerol, 0.01% Tween 80

Contains

no preservative

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Ambient

Product Specific Information

Reconstitution: Dissolve the protein in sterile double distilled water to a concentration of 0.2 mg/mL or lower. It is recommended that the protein be aliquoted and be used as soon as possible. Store aliquots under sterile conditions at -20°C. Avoid repeated freeze-thaw cycles.

Target Information

Influenza viruses consists of three types (Influenza A, B, and C), which make up three of the five genera in the Orthomyxoviridae family. Commonly know as the flu, influenza strains can affect both birds and mammals. Influenza strains form roughly spherical particles and are similar in composition with viral envelope containing two main types of glycoproteins (hemagglutinin and neuraminidase), wrapped around the central core of the viral RNA genome and other viral proteins that package and protect the RNA. Unusually for a virus, the influenzavirus genome is not a single piece of nucleic acid; rather a seven to eight segmented fragments of negative-sense RNA with each fragment expressing different viral proteins. Influenza spreads seasonally, resulting in about three to five million yearly cases of severe illness and about 250,000 to 500,000 deaths annually or into the millions in pandemic years.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.