Thermo Fisher Scientific MHC Class I (H-2Kd/H-2Dd) Monoclonal Antibody (34-1-2S), NovaFluor Blue 510, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Flow)
• Tested Dilution: 0.4 µg/test

Product Specifications

Additional Formats

• Unconjugated
• APC
• Biotin
• eFluor 450
• FITC
• Functional Grade
• NovaFluor Blue 585
• NovaFluor Blue 610-70S
• NovaFluor Blue 660-120S
• NovaFluor Red 710
• NovaFluor Yellow 570
• NovaFluor Yellow 660
• PE
• PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The 34-1-2S monoclonal antibody reacts with mouse MHC class I molecules H-2Kd and H-2Dd. It also cross-reacts with Kb, s, r, q, and p haplotypes.
This antibody is validated for flow cytometric analysis of mouse splenocytes and can be used at ≤0.4 µg per test (in 100 µL final volume). Cell concentration may vary from 10⁵–10⁸ cells/test and should be optimized empirically.

Each product includes:

• 1 vial of NovaFluor conjugate
• 1 vial of CellBlox Plus Blocking Buffer

Important Notes:

• NovaFluor dyes are not compatible with DNA intercalating viability dyes (e.g., propidium iodide, 7-AAD, DAPI).
• Use Invitrogen LIVE/DEAD Fixable Dead Cell Stains for viability analysis with NovaFluor dyes.
• CellBlox Plus Blocking Buffer (Cat. No. C001T06F01) is required for optimal staining.
  • Add 5 µL per sample before combining with cells.
  • For single-color controls: use 5 µL per 100 µL cell sample (10³–10⁸ cells).

NovaFluor conjugates utilize Phiton™ technology with engineered nucleic acid dye structures for optimized fluorescence performance and reduced spectral spillover.

• Excitation: 496 nm
• Emission: 511 nm
• Laser: 488 nm (Blue)

Target Information

MHC Class I molecules are involved in antigen presentation to CD3/TCR and CD8-expressing T cells.
These antibodies are valuable tools in Immunopeptidomics, aiding in neoantigen identification and characterization via HPLC-tandem MS analysis.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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