Thermo Fisher Scientific Phospho-E2F1 (Ser375) Polyclonal Antibody
E2F1 단백질의 Ser375 인산화 부위를 인식하는 Rabbit Polyclonal 항체로, Human, Mouse, Rat 시료에 반응. Western blot 및 ELISA에 적합하며, 고순도 Protein A 정제. 세포주기 및 종양억제 관련 연구에 활용 가능.
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Thermo Fisher Scientific Phospho-E2F1 (Ser375) Polyclonal Antibody
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic phosphopeptide derived from human E2F1 around the phosphorylation site of Ser375 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of the cell cycle and the action of tumor suppressor proteins, and is also a target of the transforming proteins of small DNA tumor viruses.
E2F proteins contain several evolutionarily conserved domains, including:
- DNA binding domain
- Dimerization domain (interacts with DP proteins)
- Transactivation domain enriched in acidic amino acids
- Tumor suppressor protein association domain within the transactivation domain
E2F1, along with E2F2 and E2F3, also possesses a cyclin binding domain. It binds preferentially to retinoblastoma protein (pRB) in a cell-cycle dependent manner and can mediate both cell proliferation and p53-dependent or independent apoptosis.
For Research Use Only.
Not for use in diagnostic procedures. Not for resale without express authorization.
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