Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647
Alexa Fluor Plus 647로 표지된 Goat anti-Mouse IgG (H+L) 고도로 교차흡착된 2차 항체로, 높은 특이성과 낮은 배경 신호를 제공하며 WB 및 ICC/IF에 적합합니다. 다양한 형광 및 효소 표지 형식으로 제공되며 연구용으로만 사용됩니다.
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Thermo Fisher Scientific Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 647
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 0.05–0.2 µg/mL |
| Immunocytochemistry (ICC/IF) | 1–10 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Mouse |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Secondary Antibody |
| Immunogen | Gamma Immunoglobins Heavy and Light chains |
| Conjugate | Alexa Fluor™ Plus 647 |
| Excitation / Emission Max | 658 / 675 nm |
| Form | Liquid |
| Concentration | 2 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | Proprietary buffer, pH 6.5 |
| Contains | 0.016% Methylisothiazolone, 0.016% Bromonitrodioxane |
| Storage Conditions | 4°C, store in dark |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_2633277 |
| Target | IgG |
| Cross Adsorption | Against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG and human serum |
| Antibody Form | Whole Antibody |
Product Specific Information
To minimize cross-reactivity, the goat anti-mouse IgG whole antibodies have been pre cross-adsorbed against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG, and human serum.
Cross-adsorption is a purification step that increases antibody specificity, reducing background staining and cross-reactivity.
The secondary antibody solution passes through column matrices containing immobilized serum proteins from potentially cross-reactive species. Highly specific antibodies are collected after multiple passages, resulting in a highly cross-adsorbed preparation.
Usage Note:
Centrifuge the conjugate solution briefly before use and use only the supernatant to minimize nonspecific background staining. Optimal dilution should be determined empirically for each application.
Target Information
Anti-Mouse secondary antibodies are affinity-purified with well-characterized specificity for mouse immunoglobulins.
They are useful for detection, sorting, or purification of target molecules and provide increased sensitivity through signal amplification.
Secondary antibodies are typically generated by immunizing host animals with pooled immunoglobulins from the target species and can be further purified or modified for enhanced specificity.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Available Conjugate Formats
- Alexa Fluor 350, 488, 546, 555, 568, 594, 633, 635, 647, 660, 680, 790
- Alexa Fluor Plus 405, 488, 555, 594, 680, 800
- AP, Biotin, DSB-X Biotin, FITC, HRP, TRITC
- Custom conjugation available upon request
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