Thermo Fisher Scientific LSD1 Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 365-515 of human KDM1 (NP_0558282) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:LSD1,uniProtId:O60341-1,ncbiNodeId:9606,antigenRange:365-515,antigenLength:852,antigenImageFileName:PA5-120127_LSD1_O60341-1_Rabbit.svg,antigenImageFileNamePDP:PA5-120127_LSD1_O60341-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.89 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2913699

Product Specific Information

Positive test controls include: MCF7, 293T. The target is usually found in the following locations: Nucleus.

Immunogen sequence: ANGQAVPKEK DEMVEQEFNR LLEATSYLSH QLDFNVLNNK PVSLGQALEV VIQLQEKHVK DEQIEHWKKI VKTQEELKEL LNKMVNLKEK IKELHQQYKE ASEVKPPRDI TAEFLVKSKH RDLTALCKEY DELAETQGKL EEKLQELEAN P

Target Information

LSD1 is a histone demethylase that specifically demethylates Lys-4 of histone H3, a specific tag for epigenetic transcriptional activation, thereby acting as a corepressor. LSD1 contains a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. It acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. LSD1 demethylates both mono- and tri-methylted Lys-4 of histone H3. This protein may play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3 Lys-4 on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. It may also demethylate Lys-9 of histone H3, a specific tag for epigenetic transcriptional repression, thereby leading to derepression of androgen receptor target genes. Mutations affecting this gene can result in cleft palate, psychomotor retardation, and distintive facial features.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.