Thermo Fisher Scientific Villin Recombinant Rabbit Monoclonal Antibody (JU34-75)
Villin 단백질을 인식하는 토끼 유래 재조합 단클론 항체로, Western blot, IHC, ICC, Flow Cytometry 등에 적합합니다. 인간 시료에 반응하며, Protein A로 정제된 액상형 제품입니다. Villin은 위장관 및 신장 상피세포의 미세융모 구조 연구에 유용한 마커입니다.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:500–1:2,000 |
| Flow Cytometry (Flow) | 1:50–1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Rabbit / IgG |
| Expression System | HEK293 cells |
| Class | Recombinant Monoclonal |
| Type | Antibody |
| Clone | JU34-75 |
| Immunogen | Synthetic peptide within Human villin1 (aa 176–225) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | TBS, pH 7.4, with 40% glycerol, 0.05% BSA |
| Contains | 0.05% sodium azide |
| Storage Conditions | -20°C, avoid freeze/thaw cycles, store in dark |
| Shipping Conditions | Wet ice |
| RRID | AB_2848528 |
Product Specific Information
Positive Controls:
Mouse colon tissue lysate, human small intestine tissue lysate, human colon tissue lysate, rat kidney tissue lysate, Hela, HepG2, LOVO, human colon cancer tissue, human kidney, mouse colon tissue.
Target Information
Villin is a cytoskeletal protein associated with the microvillar actin core bundle of gastrointestinal and renal brush borders. It interacts with actin in a Ca²⁺ and phosphoinositide-regulated manner. Villin serves as a specific marker for adenocarcinomas of the gastrointestinal tract and pancreas, and is also expressed in some Merkel cell tumors and adenocarcinomas of the lung, prostate, ovary, and kidney.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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