Thermo Fisher Scientific Villin Recombinant Rabbit Monoclonal Antibody (JU34-75)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:500-1:2,000

-

Immunohistochemistry (Paraffin) (IHC (P))

1:50-1:200

-

Immunocytochemistry (ICC/IF)

1:500-1:2,000

-

Flow Cytometry (Flow)

1:50-1:100

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

JU34-75

Immunogen

Synthetic peptide within Human villin1 aa 176-225 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:Villin,uniProtId:P09327-1,ncbiNodeId:9606,antigenRange:176-225,antigenLength:827,antigenImageFileName:MA5-34620_Villin_P09327-1_Rabbit.svg,antigenImageFileNamePDP:MA5-34620_Villin_P09327-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

TBS, pH 7.4, with 40% glycerol, 0.05% BSA

Contains

0.05% sodium azide

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles, store in dark

Shipping conditions

Wet ice

RRID

AB_2848528

Product Specific Information

Positive Control: Mouse colon tissue lysate, human small intestine tissue lysate, human colon tissue lysate, rat kidney tissue lysate, Hela, HepG2, LOVO, human colon cancer tissue, human kidney, mouse colon tissue.

Target Information

Villin is part of cytoskeleton associated with the microvillar actin core bundle of gastrointestinal and renal brush border. It can interact with actin in a Ca2+ and phosphoinositide-regulated manner. Villin is a very specific marker for adenocarcinomas of the gastrointestinal tract and the pancreas. It is also expressed in some Merkel cell tumor and adenocarcinoma of the lung, prostate, ovarian and kidney.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.