Thermo Fisher Scientific IFN gamma Monoclonal Antibody (3F1E3)
인간 IFN-γ를 검출하는 마우스 모노클로날 항체로, ELISA 및 Western blot에 최적화됨. 고순도 Protein G 정제, 1 mg/mL 농도, 비접합형 액상 제형. 인간 시료에 특이적이며 장기 보관 시 -20°C에서 안정적.
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Thermo Fisher Scientific IFN gamma Monoclonal Antibody (3F1E3)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| ELISA | 1:10,000 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 3F1E3 |
| Immunogen | Purified recombinant fragment of human IFN gamma expressed in E. coli |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1.0 mg/mL |
| Purification | Protein G |
| Storage Buffer | PBS |
| Contains | 0.03% sodium azide |
| Storage Conditions | Store at 4°C short term; for long term, store at -20°C avoiding freeze/thaw cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
| RRID | AB_11006619 |
Product Specific Information
MA5-14762 targets IFN-gamma in indirect ELISA and WB applications and shows reactivity with human samples.
The immunogen is a purified recombinant fragment of human IFN-gamma expressed in E. coli.
Target Information
IFN gamma (Interferon gamma, Type II interferon) is a macrophage activation factor produced primarily by T-lymphocytes and NK cells in response to antigens and cytokines.
It is a dimeric glycoprotein consisting of two 146 amino acid subunits (~45 kDa as a homodimer).
On SDS-PAGE, IFN gamma appears as 25, 20, and minor 15.5 kDa bands due to differential glycosylation.
Human IFN gamma shows species-specific activity and does not cross-react with mouse IFN gamma.
Biological Functions:
- Antiviral and tumor antiproliferative activity
- Induction of MHC class I and II
- Macrophage activation
- Enhancement of immunoglobulin secretion by B lymphocytes
- Cytokine regulation via JAK-STAT pathway
- Upregulated by IL-2, FGF basic, EGF; downregulated by vitamin D3 or DMN
- Mutations associated with aplastic anemia
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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