Thermo Fisher Scientific Human PIK3CA E542K/PIK3R1 (p110 alpha E542K/p85 alpha), His Tag Recombinant Protein

Applications

Tested Dilution

Publications

Kinase Assay (KA)

Assay-dependent

-

Product Specifications

Species

Human

Expression System

Insect cells

Amino acid sequence

Full length

Tag

His-tag

Molecular weight

125.4-84.7 kDa

Class

Recombinant

Type

Protein

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

See Label

Purification

purified

Storage buffer

50mM sodium phosphate, pH 7, with 300mM NaCl, 0.25mM DTT, 0.1mM PMSF, 150mM Imidazole, 25% glycerol

Contains

no preservative

Storage conditions

-80° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Dry ice

Product Specific Information

For maximum recovery please spin prior to use. Unless noted below, aliquots of the 5 µg, 10 µg and 20 µg sizes of kinase are not recommended as materials can be used in original packaging until exhausted. For larger sizes, the number of freeze/thaws may be reduced by preparing aliquots, aliquots below 20 µL are not recommended. Please never store a kinase diluted. If properly stored at -80C, this product is guaranteed for 6 months from date of purchase.

This PIK3CA E542K/PIK3R1 (p110 alpha E542K/p85 alpha) recombinant human protein (full length) was expressed in insect cells.

PIK3CA E542K/PIK3R1 (p110 alpha E542K/p85 alpha) recombinant human protein is also available in our SelectScreen™ Kinase Profiling Service.

• Validated for use with the Adapta™ assay
• Available in multiple pack sizes for convenient use
• Also available in our SelectScreen Kinase Profiling Service

Protein Form: Full Length, Recombinant, Active, Mutant

The gene accession number for PIK3CA (p110α) is NP_006209.2 and for PIK3R1 (p85α) is NP_852664.1.

Target Information

PIK3CA E542K/PIK3R1 (p110 alpha E542K/p85 alpha) lipid kinase is comprised of a 110 kDa catalytic subunit and an 85 kDa regulatory subunit. A number of isoforms of the 110 kDa catalytic subunit and the 85 kDa regulatory subunit exist in cells. PIK3CA is frequently mutated or amplified in a variety of cancers including ovarian and colon and this kinase is one of the PI3K mutants. PIK3CA gene copy number is increased in over 30% of ovarian cancers and this leads to increased PI3-kinase activity. Furthermore, the activity of PIK3CA is essential for vascular development and inactivation of PIK3CA leads to severe defects in angiogenic sprouting and vascular remodeling.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.