Thermo Fisher Scientific beta Actin Monoclonal Antibody (BA3R), DyLight 550

Applications

Tested Dilution

Publications

Western Blot (WB)

-

View 5 publications 5 publications

Immunocytochemistry (ICC/IF)

1:20-1:50

-

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rabbit, Rat

Published species

Human, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

BA3R

Immunogen

Beta-actin N-terminal peptide if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:beta Actin,uniProtId:P60709-1,ncbiNodeId:9606,antigenRange:1,antigenLength:375,antigenImageFileName:MA5-15739-D550_beta_Actin_P60709-1_House_mouse.svg,antigenImageFileNamePDP:MA5-15739-D550_beta_Actin_P60709-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

DyLight™ 550 DyLight™ 550 DyLight™ 550

View additional formats

• Unconjugated
• Biotin
• DyLight 488
• DyLight 650
• DyLight 680
• DyLight 800 4X PEG
• HRP
• Request custom conjugation

Excitation/Emission Max

556/570 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS with proprietary stabilizer

Contains

0.02% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537663

Product Specific Information

MA5-15739-D550 has been successfully used for Immunofluorescence. DyLight 550 has an excitation/emission of 562/576nm.

For visualization of actin filaments by immunofluorescence, fixation and permeabilization with methanol is required.

Target Information

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.