Thermo Fisher Scientific beta Actin Loading Control Monoclonal Antibody (BA3R), DyLight 488

Applications

Tested Dilution

Publications

Western Blot (WB)

1:1,000

View 84 publications 84 publications

Immunocytochemistry (ICC/IF)

1:20 - 1:100

-

Product Specifications

Species Reactivity

Chicken, Human, Mouse, Rabbit, Rat

Published species

Guinea pig, Human, Mouse, Plant, Rabbit, Rat

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

BA3R

Immunogen

Beta-actin N-terminal peptide. if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:beta Actin,uniProtId:P60709-1,ncbiNodeId:9606,antigenRange:1,antigenLength:375,antigenImageFileName:MA5-15739-D488_beta_Actin_P60709-1_House_mouse.svg,antigenImageFileNamePDP:MA5-15739-D488_beta_Actin_P60709-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

DyLight™ 488 DyLight™ 488 DyLight™ 488

View additional formats

• Unconjugated
• Biotin
• DyLight 550
• DyLight 650
• DyLight 680
• DyLight 800 4X PEG
• HRP
• Request custom conjugation

Excitation/Emission Max

492/519 nm View spectra

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

sodium bicarbonate

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2537662

Product Specific Information

MA5-15739-D488 detects beta actin from human, mouse, rat, rabbit, and chicken samples.

MA5-15739-D488 has been successfully used in immunofluorescence. DyLight 488 has an excitation/emission of 493/518 nm.

For visualization of actin filaments by immunofluorescence, fixation and permeabilization with methanol is required.

Target Information

Beta actin is one of six actin isoforms which have been identified. Beta actin is a non-muscle cytoskeletal protein in all human cell types and is involved in cell motility, structure, and integrity. There are six different actin isoforms in human. The beta isoform of actin, along with gamma actin, coexist in most cell types as components of the cyto-skeleton. Beta actins are cytoplasmic proteins ubuquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of two stranded helix. Actins are highly conserved proteins that are involved in cell motility, structure and integrity. Because beta actin is ubiquitously expressed in all eukaryotic cells, it is frequently used as a loading control for assays involving protein detection, such as Western blotting. Antibodies to beta-actin provide a specific and useful tool in studying the intracellular distribution of beta-actin and the static and dynamic aspects of the cytoskeleton.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.