Thermo Fisher Scientific Phosphotyrosine Monoclonal Antibody (13F9)
Phosphotyrosine 특이적 Monoclonal Antibody (Clone 13F9)로 Western blot, ELISA, IP에 사용 가능. Mouse IgG1, kappa 형식이며 phosphotyrosine에 높은 특이성과 낮은 교차반응. 연구용으로 세포 신호전달 및 인산화 단백질 분석에 적합.
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Thermo Fisher Scientific Phosphotyrosine Monoclonal Antibody (13F9)
Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| ELISA (ELISA) | 1:4,000–1:20,000 |
| Immunoprecipitation (IP) | 1:500–1:2,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Mouse / IgG1, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Clone | 13F9 |
| Immunogen | Phosphotyrosine conjugated KLH |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | Not Determined |
| Storage Buffer | Ascites |
| Contains | 0.01% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
This antibody is clarified ascites produced in Balb/c mice using clone 13F9. Reactivity is specific for phosphotyrosine with minimal cross-reactivity against phosphoserine or phosphothreonine.
Target Information
Tyrosine phosphorylation plays a key role in signal transduction from transmembrane receptors and oncogene tyrosine kinases. It is a primary mechanism of cell cycle progression and transformation.
Antibodies specific to phosphorylated tyrosine residues are invaluable for studying tyrosine-phosphorylated proteins and their biochemical pathways.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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