Thermo Fisher Scientific RALBP1 Monoclonal Antibody (OTI1E2), TrueMAB
RALBP1 단백질을 인식하는 Mouse monoclonal antibody로, Western blot, IHC, ICC, IP에 사용 가능. HEK293T 세포에서 발현된 full-length human RALBP1 항원을 면역원으로 제작. Lyophilized 형태, PBS buffer에 8% trehalose 포함, -20°C 보관.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Immunoprecipitation (IP) | 4 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI1E2 |
| Immunogen | Full length human recombinant protein of human RALBP1 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL of distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (e.g., Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
RALBP1 specifically activates hydrolysis of GTP bound to RAC1 and CDC42, but not RALA.
It mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX), serving as a major transporter of glutathione conjugates and DOX in erythrocytes.
Acts as a scaffold protein forming endocytotic complexes during interphase and interacts with CDC2 to regulate endocytosis.
One of its substrates is EPN1/Epsin.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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