Thermo Fisher Scientific ATG16L Polyclonal Antibody
ATG16L 단백질을 인식하는 Thermo Fisher Scientific의 Rabbit Polyclonal Antibody. Western blot, IHC, ICC, ELISA 등 다양한 응용 가능. Human, Mouse, Rat 반응성. 고순도 Protein A 정제 및 안정적 액상 형태로 제공.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:300 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:400 |
| Immunohistochemistry (Frozen) (IHC (F)) | 1:100–1:500 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1:500–1:1,000 |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | KLH conjugated synthetic peptide derived from human ATG16A, amino acids 501–607 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Protein A |
| Storage Buffer | 0.01M TBS, pH 7.4, with 50% glycerol, 1% BSA |
| Contains | 0.02% ProClin 300 |
| Storage Conditions | -20°C |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Target Information
Autophagy related protein 16L is encoded by the ATG16L gene located on chromosome 2 in humans. Also known as WD30 or IBD10 (Inflammatory Bowel Disease 10).
ATG12 and ATG5 conjugate with each other and oligomerize with ATG16L to form an 800 kDa complex called the Atg16L complex, which facilitates coupling or lipidation of LC3 to phosphatidylethanolamine (PE).
This ubiquitin-like conjugation system is a widely used marker for autophagy. The ATG16L complex, along with other ATGs such as ATG18 and ATG21, prevents premature cleavage of ATG8-PE by the cysteine protease Atg4 until the autophagosome is complete.
For Research Use Only.
Not for use in diagnostic procedures or resale without express authorization.
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