Thermo Fisher Scientific ATG3 Monoclonal Antibody (OTI3H1), TrueMAB
ATG3 단백질을 인식하는 Mouse monoclonal antibody로 Western blot, ICC/IF, Flow cytometry에 적합. Human 시료 반응성. Lyophilized 형태로 제공되며, PBS buffer(8% trehalose) 보존. 연구용으로 autophagy 관련 연구에 유용.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:500–1:2,000 |
| Immunocytochemistry (ICC/IF) | 1:100 |
| Flow Cytometry (Flow) | 1:100 |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI3H1 |
| Immunogen | Full length human recombinant protein of human ATG3 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
ATG3 (APG3) is a conjugating enzyme essential for APG8 lipidation, a key step in autophagy initiation. Autophagy is a bulk degradation process of cellular proteins through the autophagosomal-lysosomal pathway, crucial for growth control and nutrient preservation under starvation.
Defects in autophagy may occur in tumor cells. ATG3 acts as an E2-like enzyme, catalyzing the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, which is critical for autophagosome formation.
This process is negatively regulated by TOR (Target of Rapamycin) via phosphorylation of autophagy protein APG1.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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