Thermo Fisher Scientific ATG3 Monoclonal Antibody (OTI3H1), TrueMAB

Applications and Tested Dilution

Product Specifications

Product Specific Information

For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).

Target Information

ATG3 (APG3) is a conjugating enzyme essential for APG8 lipidation, a key step in autophagy initiation. Autophagy is a bulk degradation process of cellular proteins through the autophagosomal-lysosomal pathway, crucial for growth control and nutrient preservation under starvation.
Defects in autophagy may occur in tumor cells. ATG3 acts as an E2-like enzyme, catalyzing the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, which is critical for autophagosome formation.
This process is negatively regulated by TOR (Target of Rapamycin) via phosphorylation of autophagy protein APG1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.