Thermo Fisher Scientific RBM25 Polyclonal Antibody
Thermo Fisher Scientific RBM25 Polyclonal Antibody는 인간 및 마우스 시료에서 RNA 결합 단백질 RBM25를 검출하기 위한 고품질 항체입니다. Western blot, ICC/IF, ELISA에 적합하며, 고순도 친화 크로마토그래피 정제 및 안정적 PBS/glycerol 저장 버퍼를 사용합니다.
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Applications
| Application | Tested Dilution | Publications |
|---|---|---|
| Western Blot (WB) | 1:200–1:2,000 | View 1 publication |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 | – |
| ELISA | 1 µg/mL | – |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse |
| Published Species | Not Applicable |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 614–843 of human RBM25 (NP_0670621) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.87 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.01% thimerosal |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2806104 |
Product Specific Information
Immunogen Sequence:
KPTLRPISSA PSVSSASGNA TPNTPGDESP CGIIIPHENS PDQQQPEEHR PKIGLSLKLG ASNSPGQPNS VKRKKLPVDS VFNKFEDEDS DDVPRKRKLV PLDYGEDDKN ATKGTVNTEE KRKHIKSLIE KIPTAKPELF AYPLDWSIVD SILMERRIRP WINKKIIEYI GEEEATLVDF VCSKVMAHSS PQSILDDVAM VLDEEAEVFI VKMWRLLIYE TEAKKIGLVK
Positive Samples: DU145, OVCAR3, HepG2, Jurkat, NIH/3T3, mouse brain, mouse liver
Cellular Location: Cytoplasm, Nucleus speckle
Target Information
RNA-binding protein that acts as a regulator of alternative pre-mRNA splicing. Involved in apoptotic cell death through regulation of the apoptotic factor BCL2L1 isoform expression. Modulates the ratio of proapoptotic BCL2L1 isoform S to antiapoptotic BCL2L1 isoform L mRNA expression. When overexpressed, stimulates proapoptotic BCL2L1 isoform S 5'-splice site (5'-ss) selection, whereas its depletion causes accumulation of antiapoptotic BCL2L1 isoform L. Promotes BCL2L1 isoform S 5'-ss usage through the 5'-CGGGCA-3' RNA sequence. Association with LUC7L3 promotes U1 snRNP binding to a weak 5' ss in a 5'-CGGGCA-3'-dependent manner. Binds to the exonic splicing enhancer 5'-CGGGCA-3' RNA sequence located within exon 2 of the BCL2L1 pre-mRNA. Also involved in the generation of an abnormal and truncated splice form of SCN5A in heart failure.
⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm.
For more information, visit www.P65Warnings.ca.gov.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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