Thermo Fisher Scientific TET2 Monoclonal Antibody (CL6873)

Applications

Tested Dilution

Publications

Western Blot (WB)

1 µg/mL

-

Immunohistochemistry (Paraffin) (IHC (P))

1:20-1:50

-

Immunocytochemistry (ICC/IF)

2-10 µg/mL

-

Product Specifications

Species Reactivity

Human

Host/Isotype

Mouse / IgG2b

Class

Monoclonal

Type

Antibody

Clone

CL6873

Immunogen

Synthetic peptide corresponding to Human TET2 if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:TET2,uniProtId:Q6N021-1,ncbiNodeId:9606,antigenRange:931-949,antigenLength:2002,antigenImageFileName:MA5-31442_TET2_Q6N021-1_House_mouse.svg,antigenImageFileNamePDP:MA5-31442_TET2_Q6N021-1_House_mouse_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

1 mg/mL

Purification

Protein A

Storage buffer

PBS, pH 7.2, with 40% glycerol

Contains

0.02% sodium azide

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Wet ice

RRID

AB_2787078

Product Specific Information

Immunogen sequence: PDQGGSHTQT PPQKDTQKH

Target Information

TET2 is a dioxygenase that catalyzes the conversion of the modified genomic base 5-methylcytosine (5mC) into 5-hydromethylcytosine (5hmC) and plays a key role in active DNA demethylation. TET2 has a preference for 5-hydroxymethylcytosine in CpG motifs. It mediates subsequent conversion of 5hmC into 5-formylcytosine (5fC) and conversion of 5fC to 5-carboyxlcytosine (5caC). Conversion of 5mC into 5hmC, 5fC, and 5caC probably constitutes the first step in cytonsine demethylation. In addition to TET2`s role in DNA demethylation, it is also involved in the recruitment of the O-GlcNAc transferase OGT to CpG-rich transcription start sites of active genes, thereby promoting histone H2B GlcNAcylation by OGT. Mutations in the genes can result in Polycythemia vera and Myelodysplastic syndrome.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.