Thermo Fisher Scientific UBE2O Polyclonal Antibody
Thermo Fisher Scientific의 UBE2O Polyclonal Antibody는 인간 및 마우스 시료에 반응하며, WB 및 IP에 적합합니다. 항원 친화 크로마토그래피로 정제된 비결합 항체로, 안정적인 액상 형태로 제공됩니다. 연구용으로 TRAF6 및 BMP7 신호 조절 연구에 활용됩니다.
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Applications
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:2,000–1:10,000 |
| Immunoprecipitation (IP) | 2–10 µg/mg lysate |
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Region between residue 725 and 775 of Human ubiquitin-conjugating enzyme E2O |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.20 mg/mL |
| Purification | Antigen affinity chromatography |
| Storage buffer | TBS, pH 7.0–8.0, with 0.1% BSA |
| Contains | 0.09% sodium azide |
| Storage conditions | 4°C |
| Shipping conditions | Wet ice |
Product Specific Information
The recommended shelf life for this product is 1 year from date of receipt.
Target Information
E2/E3 hybrid ubiquitin-protein ligase that displays both E2 and E3 ligase activities and mediates monoubiquitination of target proteins (PubMed:23455153, PubMed:24703950).
Negatively regulates TRAF6-mediated NF-kappa-B activation independently of its E2 activity (PubMed:23381138).
Acts as a positive regulator of BMP7 signaling by mediating monoubiquitination of SMAD6, thereby regulating adipogenesis (PubMed:23455153).
Mediates monoubiquitination at different sites of the nuclear localization signal (NLS) of BAP1, leading to cytoplasmic retention of BAP1.
Also able to monoubiquitinate the NLS of other chromatin-associated proteins, such as INO80 and CXXC1, affecting their subcellular location (PubMed:24703950).
Acts as a regulator of retrograde transport by assisting the TRIM27:MAGEL2 E3 ubiquitin ligase complex to mediate Lys-63-linked ubiquitination of WASHC1, leading to promote endosomal F-actin assembly (PubMed:23452853).
[UniProt]
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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