Thermo Fisher Scientific CDC123 Monoclonal Antibody (OTI5C7), TrueMAB
CDC123 단백질을 인식하는 Mouse IgG2b 단일클론 항체로 Western blot 및 Immunocytochemistry에 최적화됨. HEK293T 세포에서 발현된 full-length human recombinant CDC123을 면역원으로 사용. Lyophilized 형태로 제공되며, 재구성 및 결합 실험에 적합. 연구용으로만 사용 가능.
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Applications
Western Blot (WB)
- Tested Dilution: 1:400–1:4,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Clone | OTI5C7 |
| Immunogen | Full-length human recombinant protein of human CDC123 produced in HEK293T cell |
| Conjugate | Unconjugated |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS, pH 7.3, with 8% trehalose |
| Contains | No preservative |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
For reconstitution, add 100 µL distilled water to achieve a final antibody concentration of approximately 1 mg/mL.
For conjugation experiments using this carrier-free antibody, perform an additional desalting step (recommended: Zeba Spin Desalting Columns, 7K MWCO, 0.5 mL, Product #89882).
Target Information
The BCKDHA gene encodes the E1-alpha subunit of the branched-chain alpha-keto acid dehydrogenase complex (BCKD), which is involved in branched-chain amino acid (BCAA) oxidation.
The complex comprises three catalytic components:
- A heterotetrameric (α2-β2) decarboxylase (E1)
- A homo-24-meric dihydrolipoyl transacylase
- A homodimeric dihydrolipoamide dehydrogenase
E1 is a thiamine pyrophosphate (TPP)-dependent enzyme catalyzing the irreversible first step in BCAA oxidation.
The BCKDHB gene encodes the beta subunit of E1.
Additionally, the complex includes two regulatory enzymes, a kinase and a phosphorylase.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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