Thermo Fisher Scientific FGF1 Polyclonal Antibody, Biotin, PeproTech

Applications and Tested Dilution

Product Specifications

Product Specific Information

AA Sequence of recombinant protein:
MFNLPPGNYK KPKLLYCSNG GHFLRILPDG TVDGTRDRSD QHIQLQLSAE SVGEVYIKST ETGQYLAMDT DGLLYGSQTP NEECLFLERL EENHYNTYIS KKHAEKNWFV GLKKNGSCKR GPRTHYGQKA ILFLPLPVSS D.

Preparation:
Produced from sera of rabbits immunized with highly pure Recombinant Human FGF-acidic. Anti-Human FGF-acidic-specific antibody was purified by affinity chromatography and then biotinylated.

Sandwich ELISA:
To detect hFGF-acidic by sandwich ELISA (using 100 µL/well antibody solution), a concentration of 0.25–1.0 µg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with PeproTech Polyclonal Anti-Human FGF-acidic (500-P17) as a capture antibody, allows the detection of at least 0.2–0.4 ng/well of Recombinant hFGF-acidic.

Western Blot:
To detect Human FGF-acidic by Western Blot analysis, this antibody can be used at a concentration of 0.1–0.2 µg/mL. Used in conjunction with compatible secondary reagents, the detection limit for Recombinant Human FGF-acidic is 1.5–3.0 ng/lane, under either reducing or non-reducing conditions.

Package Information:
500-P17BT-1MG will be provided as 2 × 500 µg.

Target Information

FGF1 is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in various biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, suggesting its role in organogenesis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.