Thermo Fisher Scientific ALDH1A1 Recombinant Rabbit Monoclonal Antibody (3X6B2)

Applications

Tested Dilution

Publications

Western Blot (WB)

1:50,000-1:200,000

-

Immunocytochemistry (ICC/IF)

1:100-1:800

-

ELISA (ELISA)

1 µg/mL

-

Immunoprecipitation (IP)

0.5 µg-4 µg antibody for 200 µg-400 µg extracts of

-

Product Specifications

Species Reactivity

Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Expression System

HEK293 cells

Class

Recombinant Monoclonal

Type

Antibody

Clone

3X6B2

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 250-350 of human ALDH1A1 (NP_0006802) if (typeof window.$mangular === undefined || !window.$mangular) { window.$mangular = {}; } $mangular.antigenJson = \[{targetFamily:ALDH1A1,uniProtId:P00352-1,ncbiNodeId:9606,antigenRange:250-350,antigenLength:501,antigenImageFileName:MA5-42586_ALDH1A1_P00352-1_Rabbit.svg,antigenImageFileNamePDP:MA5-42586_ALDH1A1_P00352-1_Rabbit_PDP.jpeg,sortOrder:1}\]; $mangular.isB2BCMGT = false; $mangular.isEpitopesModalImageMultiSizeEnabled = true;

View immunogen .st0{fill:#FFFFFF;} .st1{fill:#1E8AE7;}

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity Chromatography

Storage buffer

PBS, pH 7.3, with 0.05% BSA, 50% glycerol

Contains

0.05% ProClin 300

Storage conditions

-20° C, Avoid Freeze/Thaw Cycles

Shipping conditions

Wet ice

RRID

AB_2911727

Product Specific Information

Positive test controls include: HepG2, A-549, K-562, Mouse lung, Mouse testis, Rat lung, Rat liver, Rat kidney. The target is usually found in the following locations: Cytoplasm.

Immunogen sequence: VGKLIKEAAG KSNLKRVTLE LGGKSPCIVL ADADLDNAVE FAHHGVFYHQ GQCCIAASRI FVEESIYDEF VRRSVERAKK YILGNPLTPG VTQGPQIDKE Q

Target Information

ALDH1A1 belongs to the aldehyde dehydrogenases family of proteins. Aldehyde dehydrogenase is the second enzyme of the major oxidative pathway of alcohol metabolism. Two major liver isoforms of this enzyme, cytosolic and mitochondrial, can be distinguished by their electrophoretic mobilities, kinetic properties, and subcellular localizations. Most Caucasians have two major isozymes, while approximately 50% of Orientals have only the cytosolic isozyme, missing the mitochondrial isozyme. A remarkably higher frequency of acute alcohol intoxication among Orientals than among Caucasians could be related to the absence of the mitochondrial isozyme.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.