Thermo Fisher Scientific E2F1 Polyclonal Antibody
E2F1 단백질을 인식하는 Rabbit Polyclonal Antibody로, 인간 및 생쥐 시료에 반응합니다. Western Blot 및 Immunocytochemistry에 적합하며, 고순도 친화 크로마토그래피로 정제되었습니다. 액상 형태로 제공되며, -20°C에서 보관합니다.
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Applications
Western Blot (WB)
- Tested Dilution: 1:500–1:2,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:100–1:500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | A synthesized peptide derived from human E2F1 (Accession Q01094), corresponding to amino acid residues L342–S392 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | PBS with 50% glycerol |
| Contains | 0.02% sodium azide |
| Storage Conditions | -20°C |
| Shipping Conditions | Wet ice |
| RRID | AB_2899335 |
Product Specific Information
Antibody detects endogenous levels of total E2F1.
Target Information
The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of the cell cycle and the action of tumor suppressor proteins. It is also a target of transforming proteins of small DNA tumor viruses.
E2F proteins contain several conserved domains, including:
- DNA binding domain
- Dimerization domain (interacts with DP proteins)
- Transactivation domain (acidic amino acid-rich)
- Tumor suppressor protein association domain (within transactivation domain)
E2F1, along with E2F2 and E2F3, possesses an additional cyclin-binding domain. It binds preferentially to retinoblastoma protein (pRB) in a cell-cycle dependent manner and can mediate both cell proliferation and p53-dependent/independent apoptosis.
For Research Use Only.
Not for use in diagnostic procedures.
Not for resale without express authorization.
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