Thermo Fisher Scientific Human CDK2/cyclin O, GST Tag Recombinant Protein
Recombinant full-length human CDK2/Cyclin O protein with N-terminal GST tag for kinase assays. Expressed in baculovirus system and validated in LanthaScreen™ Kinase Binding Assay. Supplied in liquid form, purified, and stored at -80°C for stable perfor...
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Applications
- Kinase Assay (KA): Assay-dependent
Product Specifications
| 항목 | 내용 |
|---|---|
| Species | Human |
| Expression System | Baculovirus |
| Amino Acid Sequence | 1–264 |
| Tag | GST-tag |
| Molecular Weight | 60.2–64.4 kDa |
| Class | Recombinant |
| Type | Protein |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | See Label |
| Purification | Purified |
| Storage Buffer | 50 mM Tris-HCl (pH 7.5), 25% glycerol, 150 mM NaCl, 0.1 mM PMSF, 0.1 mM EDTA, 0.25 mM DTT, 10 mM glutathione |
| Contains | No preservative |
| Storage Conditions | -80°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Dry ice |
Product Specific Information
For maximum recovery, please spin prior to use.
Aliquots of 5 µg, 10 µg, and 20 µg sizes are not recommended; use the original packaging until exhausted.
For larger sizes, reduce freeze/thaw cycles by preparing aliquots (not below 20 µL).
Never store diluted kinase.
If properly stored at -80°C, guaranteed for 6 months from date of purchase.
Recombinant full-length human CDK2/Cyclin O was expressed in Sf9 insect cells using an N-terminal GST tag.
Key Features
- Validated in LanthaScreen™ Kinase Binding Assay
- Protocols available for optimized performance
- Multiple pack sizes for convenient use
Related Product: LanthaScreen™ Eu Kinase Binding Assay
Protein Form: Recombinant
Gene Accession Numbers: CDK2 (NP_001789.2), Cyclin O (NP_066970.3)
Target Information
CDK2/Cyclin O is a member of the cyclin-dependent protein kinase family complexed with Cyclin O.
Cyclin O, an isoform of the UNG gene, is localized in the nucleus and exhibits uracil-DNA glycosylase activity.
CDK2/Cyclin O protein levels increase during G1 phase and degrade during the cell cycle.
Cyclin A–CDK2 levels peak at the G1/S transition and associate with DNA during replication initiation.
Rb protein acts as a substrate for CDK2–Cyclin E and CDK2–Cyclin A in vivo.
CDK2 is activated and localized to the nucleus during late G1, S, and G2 phases.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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