Thermo Fisher Scientific R-Phycoerythrin Polyclonal Antibody, Rhodamine
R-Phycoerythrin에 특이적인 산양 다클론 항체로 Rhodamine 형광으로 표지됨. Western blot 및 면역세포염색에 사용 가능. 친화 크로마토그래피로 정제되어 높은 특이성과 재현성 제공. 573/591 nm에서 여기/방출 특성. 연구용 전용 제품.
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Applications
Western Blot (WB)
- Tested Dilution: 1:2,000–1:20,000
Immunocytochemistry (ICC/IF)
- Tested Dilution: 1:500–1:2,500
Product Specifications
| 항목 | 내용 |
|---|---|
| Species Reactivity | Chemical |
| Host / Isotype | Goat / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | This affinity purified antibody was prepared from whole goat serum produced by repeated immunizations with highly purified R-Phycoerythrin from the seaweed Gracilaria. |
| Conjugate | Rhodamine |
| Excitation / Emission Max | 573 / 591 nm |
| Form | Lyophilized |
| Concentration | 1 mg/mL |
| Purification | Affinity chromatography |
| Storage Buffer | 0.02M potassium phosphate, pH 7.2, with 0.15M NaCl, 10 mg/mL BSA |
| Contains | 0.01% sodium azide |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Ambient (domestic); Wet ice (international) |
Product Specific Information
Store vial at 4°C prior to restoration. For extended storage, aliquot contents and freeze at -20°C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature.
This product is stable for several weeks at 4°C as an undiluted liquid. Dilute only prior to immediate use.
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum and R-Phycoerythrin conjugated IgG.
This antibody will cross-react with B-Phycoerythrin. Reactivity with other phycobiliproteins is unknown.
Target Information
Anti-RPE (R-Phycoerythrin) antibodies can be used to bind conjugated primary antibody without decreasing fluorescent intensity of the RPE fluorochrome.
This method is used for separation of cells labeled with RPE-conjugated antibodies.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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