Thermo Fisher Scientific IPAB Polyclonal Antibody

Applications

Tested Dilution

Publications

Western Blot (WB)

Assay-dependent

-

ELISA (ELISA)

Assay-dependent

-

Product Specifications

Species Reactivity

Bacteria

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant Shigella flexneri IPAB protein (1-312aa)

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

3.904 mg/mL

Purification

Protein G

Storage buffer

PBS, pH 7.4, with 50% glycerol

Contains

0.03% Proclin 300

Storage conditions

-20°C or -80°C if preferred

Shipping conditions

Wet ice

RRID

AB_3091631

Target Information

Component of the type III secretion system (T3SS), also called injectisome, which is used to inject bacterial effector proteins into eukaryotic host cells (PubMed:10545510, PubMed:17367391). IpaB/SctE and IpaC/SctB are inserted into the host membrane where they form a pore and allow the translocation of effector proteins into the cytosol of target cells (PubMed:10545510, PubMed:17367391, PubMed:34809452). Interaction with IpaD/SctA at needle tips leads to the formation of the MxiH/SctF-IpaD/SctA-IpaB/SctE ternary complex, which is essential for host cell sensing (PubMed:17367391, PubMed:19433542). Interaction of IpaB/SctE with host membrane lipids promotes recruitment of IpaC/SctB at the needle tip concomitant with translocon insertion into the host membrane and type III secretion induction (PubMed:19433542).

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.