Thermo Fisher Scientific Carbonic Anhydrase IX Polyclonal Antibody
Carbonic Anhydrase IX 단백질을 인식하는 Rabbit Polyclonal 항체. Western blot, IHC, ICC/IF에 사용 가능. 열 매개 항원 회복 권장. 저산소 상태에서 발현되는 CA IX 연구에 적합. 연구용으로만 사용.
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Applications and Tested Dilutions
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:250 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:250 |
| Immunocytochemistry (ICC/IF) | 5 µg/mL |
Product Specifications
| 항목 | 내용 |
|---|---|
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Synthetic peptide conjugated to KLH derived from within residues 100–200 of Human Carbonic Anhydrase IX |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.5 mg/mL |
| Storage Conditions | Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles. |
| Shipping Conditions | Wet ice |
| RRID | AB_10982975 |
Product Specific Information
Heat mediated antigen retrieval is recommended prior to tissue staining.
Target Information
Carbonic anhydrase (CA) is an enzyme that catalyzes the rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues.
CA IX is one of the most inducible genes under hypoxia via HIF-1α, due to its stability and membrane localization.
Carbonic anhydrases play a key role in regulating pH by controlling hydrogen ion (H⁺) flux in normal tissues.
Under hypoxic conditions, inhibition of CA IX increases cell death, making CA IX a reliable histochemical marker of hypoxia.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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