Thermo Fisher Scientific GPLD1 Polyclonal Antibody
Thermo Fisher Scientific의 GPLD1 Polyclonal Antibody는 인간, 마우스, 랫트 반응성을 가지며 Western blot, IHC, ICC/IF, ELISA에 적합합니다. Rabbit IgG 폴리클로날 항체로, 정제된 액상 형태이며 -20°C 보관이 권장됩니다. 연구용으로만 사용 가능합니다.
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Applications and Tested Dilution
| Application | Tested Dilution |
|---|---|
| Western Blot (WB) | 1:100–1:500 |
| Immunohistochemistry (Paraffin) (IHC (P)) | 1:50–1:200 |
| Immunocytochemistry (ICC/IF) | 1:50–1:200 |
| ELISA | 1 µg/mL |
Product Specifications
| Specification | Description |
|---|---|
| Species Reactivity | Human, Mouse, Rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 24–160 of human GPLD1 (NP_001494.2) |
| Conjugate | Unconjugated |
| Form | Liquid |
| Concentration | 0.75 mg/mL |
| Purification | Affinity Chromatography |
| Storage Buffer | PBS, pH 7.3, with 50% glycerol |
| Contains | 0.09% sodium azide |
| Storage Conditions | -20°C, Avoid Freeze/Thaw Cycles |
| Shipping Conditions | Wet ice |
| RRID | AB_2914586 |
Product Specific Information
- Positive test controls: Human plasma, Mouse plasma, Mouse liver, Rat plasma
- Target localization: Secreted
Immunogen sequence:
CGLSTHVEIG HRALEFLQLH NGRVNYRELL LEHQDAYQAG IVFPDCFYPS ICKGGKFHDV SESTHWTPFL NASVHYIREN YPLPWEKDTE KLVAFLFGIT SHMAADVSWH SLGLEQGFLR TMGAIDFHGS YSEAHSA
Target Information
GPLD1 is expressed in numerous tissues and cells and specifically cleaves GPI-anchored proteins. Liver has the highest level of GPI-PLD expression and is the primary organ contributing to GPLD1 in the serum. GPLD1 is abundant in serum in which it associates with apolipoproteins AI and AIV. Increased serum GPLD1 is associated with insulin resistance and elevated serum triglycerides. Many surface proteins are attached to eukaryotic cell membranes via glycosylphosphatidylinositol (GPI) anchors that are covalently bound to the C-terminus of the protein. Cleavage of the GPI moiety by GPLD1 may regulate attachment of these proteins to the cell surface or their release into the extracellular environment.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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