Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), APC-eFluor 780, eBioscience

Thermo Fisher Scientific Ki-67 Monoclonal Antibody (20Raj1), APC-eFluor 780, eBioscience

Applications and Tested Dilution

• Application: Flow Cytometry (Intracellular staining)
• Tested Dilution: 5 µL (0.25 µg)/test
• Publications: View 10 publications

Product Specifications

Available Formats

• Unconjugated
• APC
• Biotin
• Brilliant UV 395 / 563 / 615 / 737 / 805
• Brilliant Violet 421 / 480 / 605 / 650 / 711 / 786
• eFluor 450 / 506 / 570 / 615 / 660
• FITC / PE / PE-Cyanine5.5 / PE-Cyanine7 / PE-eFluor 610 / PerCP-eFluor 710
• Request custom conjugation

Product Specific Information

The monoclonal antibody 20Raj1 recognizes the human Ki-67 protein, which exists in two isoforms (345 and 395 kDa) expressed in dividing cells. Ki-67 is detectable during active phases of the cell cycle (G1, S, G2, and mitosis) but absent from resting cells (G0).
During interphase, Ki-67 localizes to the nucleus and redistributes to chromosomes during mitosis, associating with heterochromatin-bound proteins such as CBX3.
In tumor studies, Ki-67 expression serves as a marker for determining the fraction of proliferating cells.
This antibody also recognizes canine Ki-67.

Applications Reported: Intracellular staining followed by flow cytometric analysis.
Applications Tested: Pre-diluted and validated for flow cytometry using the Foxp3/Transcription Factor Staining Buffer Set (Product #00-5523-00).
Use at 5 µL (0.25 µg) per test (100 µL final volume). Cell count may range from 10⁵–10⁸ cells/test.

Fluorochrome: APC-eFluor 780 (Excitation: 633–647 nm; Emission: 780 nm)
Ensure instrument compatibility with this fluorochrome.

Light Sensitivity: Protect vial and stained samples from light.
Fixation: Samples can be stored up to 3 days at 4°C in the dark using

• IC Fixation Buffer (Product #00-8222-49)
• 1-step Fix/Lyse Solution (Product #00-5333-57)

Target Information

Ki-67 is a nuclear protein expressed during late G1, S, G2, and M phases. It contains a forkhead-associated (FHA) domain that mediates association with euchromatin and nucleolar regions.
Ki-67 shows cell cycle–dependent localization: perinucleolar at G1, nuclear matrix at G2, and chromosomal during M phase.
It serves as a proliferation marker, absent in G0 cells, and correlates with tumor grade and prognosis.
Commonly used in neoplasm studies and neuronal proliferation assays.

For Research Use Only. Not for use in diagnostic procedures or resale without authorization.

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